The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be used for the establishment of molecular strategies for use in passion fruit breeding and genetics.

Em virtude da baixa variabilidade genética relatada nos plantios comerciais de mamoeiro (Carica papaya L.), o objetivo deste trabalho foi analisar a divergência genética de 32 genótipos incluindo cultivares, variedades locais, linhagens e germoplasma melhorado, utilizando a técnica de AFLP (Amplified Fragment Length Polymorphism). A matriz de distâncias genéticas foi obtida com a distância de Nei e Li, e o agrupamento foi realizado utilizando o método UPGMA (unweighted pair-method with arithmetic mean). Utilizaram-se 11 combinações de iniciadores com corte das enzimas EcoRI / MseI. Foram geradas 383 bandas polimórficas, com média de 34,8 por combinação de iniciadores. O agrupamento dos dados permitiu a formação de cinco grupos, sendo a cultivar Sunrise e a linhagem CMF-L30-08 os genótipos mais relacionados, e CMF041 (germoplasma melhorado) e CMF233 (variedade local), os mais dissimilares. As cultivares de mamoeiro mais cultivadas no Brasil, bem como quatro linhagens e três acessos de germoplasma melhorado foram agrupadas, porém não no mesmo ramo. Mesmo sendo oriunda de mutação e seleção dentro de Sunrise, a cultivar Golden ainda possui considerável variabilidade genética, uma vez que a distância genética entre estas cultivares foi de 0,329. Variabilidade adicional foi observada nas linhagens do programa de melhoramento.

Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes, and the improved germplasm (CMF041) and landrace (CMF233) the most distant. The main papaya cultivars commercially grown in Brazil, as well as four inbred lines and three improved germplasm, were clustered together, however, were not grouped in the same branch. The genetic distance between the Sunrise and Golden cultivars was 0.329, and even arising from mutation and selection within the Sunrise variety, the Golden stores considerable genetic variability. Additional variability was observed in the inbred lines derived from papaya breeding program at Embrapa Cassava and Fruits.